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pLenti_CamKIIa_mKate2
(Plasmid #96942)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 96942 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti
  • Vector type
    Lentiviral
  • Selectable markers
    Zeo marker is outside the LTRs and will not be packaged into virus.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    far-red fluorescent protein
  • Alt name
    mKate2
  • Mutation
    E87D (please see depositor comments below)
  • Promoter CaMKIIa

Cloning Information

Resource Information

Depositor Comments

Please note that mutation E87D in mKate2 was found during Addgene's quality control process. The depositor noted that this mutation does NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti_CamKIIa_mKate2 was a gift from Ricardo Dolmetsch & Oleksandr Shcheglovitov (Addgene plasmid # 96942 ; http://n2t.net/addgene:96942 ; RRID:Addgene_96942)
  • For your References section:

    SHANK3 and IGF1 restore synaptic deficits in neurons from 22q13 deletion syndrome patients. Shcheglovitov A, Shcheglovitova O, Yazawa M, Portmann T, Shu R, Sebastiano V, Krawisz A, Froehlich W, Bernstein JA, Hallmayer JF, Dolmetsch RE. Nature. 2013 Nov 14;503(7475):267-71. doi: 10.1038/nature12618. Epub 2013 Oct 16. 10.1038/nature12618 PubMed 24132240