Desmin/pAS2-1
(Plasmid #97407)

• Purpose: Desmin (aa 30 – 103) in DNA-binding domain (BD) vector, used for yeast-two hybrid
• Depositing Lab: Rita Shiang
• Publication: Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi: 10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10.

Backbone

• Vector backbone: pAS2-1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 8393
• Total vector size (bp): 8604
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Desmin
• Species: H. sapiens (human)
• GenBank ID: NM_001927
• Entrez Gene: DES (a.k.a. CDCD3, CSM1, CSM2, LGMD1D, LGMD1E, LGMD2R)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: Gal4 N-term
• 3′ sequencing primer: GAL4 3'-BD: 5'-CATAAGAAATTCGCCCGG-3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA