pGreenPuro-shRNA-Stx3S-C4
(Plasmid #99742)

• Purpose: 3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.
• Depositing Lab: Thomas Weimbs
• Publication: Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-5491. doi: 10.1074/jbc.RA117.000874. Epub 2018 Feb 23.

Backbone

• Vector backbone: pGreenPuro
• Backbone manufacturer: System Biosciences Inc.
• Backbone size w/o insert (bp): 7861
• Total vector size (bp): 7882
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Puromycin ; CopGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: shRNA against Stx3S
• gRNA/shRNA sequence: ggaagaaactgatttcactcc
• Species: H. sapiens (human)
• Promoter: H1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: AATGTCTTTGGATTTGGGAATCTTAT
• 3′ sequencing primer: TGGTCTAACCAGAGAGACCCAGTA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry