Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha.
Sugano SS, Nishihama R, Shirakawa M, Takagi J, Matsuda Y, Ishida S, Shimada T, Hara-Nishimura I, Osakabe K, Kohchi T
PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 71534 | pMpGE_En01 | Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha |
| 71535 | pMpGE_En03 | Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha |
| 71536 | pMpGE010 | For CRISPR/Cas9 genome editing in Marchantia polymorpha |
| 71537 | pMpGE011 | For CRISPR/Cas9 genome editing in Marchantia polymorpha |
| 108681 | pMpGE013 | All-in-one genome editing vector in M. polymorpha (hygromycin resistant) |
| 108682 | pMpGE014 | All-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant) |
| 108722 | pMpGE006 | Cas9 expression in M.polymorpha |