Tunable protein degradation in bacteria.
Cameron DE, Collins JJ
Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 75439 | pZA16mflon | Plasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli. |
| 75441 | pECGMC3 | Plasmid for mf-lon mediated mCherry degradation in Lactococcus lactis. Contains mcherry-pdt3 |
| 75445 | pECM1 | Plasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicate |
| 75446 | pECT1 | Plasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate |
| 75447 | pECT1B | Plasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate |
| 75448 | pECT1D | Plasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate |
| 75449 | pECT1E | Plasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate |
| 75450 | pECT3 | Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate |
| 75451 | pECT5 | Plasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate |
| 75452 | pZE27GFP | Plasmid for constitutive expression of gfp |
| 75453 | pZE27GFP0 | Plasmid for constitutive expression of gfp-pdt |
| 75454 | pZE27GFP1 | Plasmid for constitutive expression of gfp-pdt1 |
| 75455 | pZE27GFP2 | Plasmid for constitutive expression of gfp-pdt2 |
| 75456 | pZE27GFP3 | Plasmid for constitutive expression of gfp-pdt3 |
| 75457 | pZE27GFP4 | Plasmid for constitutive expression of gfp-pdt4 |
| 75458 | pZE27GFP5 | Plasmid for constitutive expression of gfp-pdt5 |
| 75459 | pZE27GFP3A | Plasmid for constitutive expression of gfp-pdt3A |
| 75460 | pZE27GFP3B | Plasmid for constitutive expression of gfp-pdt3B |
| 75461 | pZE27GFP3C | Plasmid for constitutive expression of gfp-pdt3C |
| 75462 | pZE27GFP3D | Plasmid for constitutive expression of gfp-pdt3D |
| 75463 | pZE27GFP3E | Plasmid for constitutive expression of gfp-pdt3E |
| 75464 | pZE27MC3 | Plasmid for constitutive expression of mcherry-pdt3 |
| 75465 | pECJ3 | Toggle switch plasmid containing lacI-pdt3 |
| 75466 | pECJ3B | Toggle switch plasmid containing lacI-pdt3B |
| 75467 | pECJ3D | Toggle switch plasmid containing lacI-pdt3D |