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Dynamics of epigenetic regulation at the single-cell level.
Bintu L, Yong J, Antebi YE, McCue K, Kazuki Y, Uno N, Oshimura M, Elowitz MB
Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
78099PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-insNuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).
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78101pEx1-pEF-H2B-mCherry-T2A-rTetR-EEDExpresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.
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78348pEx1-pEF-H2B-mCherry-T2A-rTetR-KRABExpresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.
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78349pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.
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78352pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-ZeoExpresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.
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78353pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4-ZeoExpresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.
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