miR-34 activity is modulated through 5'-end phosphorylation in response to DNA damage.
Salzman DW, Nakamura K, Nallur S, Dookwah MT, Metheetrairut C, Slack FJ, Weidhaas JB
Nat Commun. 2016 Mar 21;7:10954. doi: 10.1038/ncomms10954.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 78258 | psiCHECK2-miR-34 WT | psiCHECK2 dual luciferase reporter harboring a fully complementary miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene |
| 78259 | psiCHECK2-miR-34 MT | psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene |
| 78260 | psiCHECK2-let-7 WT | psiCHECK2 dual luciferase reporter harboring a fully complementary let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene |
| 78261 | psiCHECK2-let-7 MT | psiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene |