Binding of STIL to Plk4 activates kinase activity to promote centriole assembly.
Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ
J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 80264 | pFastBac GST-Plk4 | baculovirus creation plasmid for GST-Plk4 |
| 80265 | pFastBac GST-STIL | baculovirus creation plasmid for GST-STIL |
| 80266 | pcDNA5 FRT/TO MycLAP-STIL | mammalian expression plasmid for MycLAP-STIL |
| 80267 | pcDNA5 FRT/TO MycLAP-STIL 5A | mammalian expression plasmid for MycLAP-STIL 5A |
| 80268 | pcDNA5 FRT/TO MycLAP-STIL ∆CC | mammalian expression plasmid for MycLAP-STIL coiled-coil deletion |
| 80269 | pcDNA5 FRT/TO Plk4-mCherry | mammalian expression plasmid for Plk4-mCherry |
| 80270 | pcDNA5 FRT/TO Plk4 KD-mCherry | mammalian expression plasmid for kinase dead Plk4 |
| 80271 | pcDNA5 FRT/TO Plk4 ∆24-mCherry | mammalian expression plasmid for Plk4 with multi-phospho degron deleted |
| 80272 | pcDNA5 FRT/TO Plk4 KD ∆24-mCherry | mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted |
| 80273 | pFastBac GST-SAS6 | baculovirus creation plasmid for GST-SAS6 |