Screening and large-scale expression of membrane proteins in mammalian cells for structural studies.

Goehring A, Lee CH, Wang KH, Michel JC, Claxton DP, Baconguis I, Althoff T, Fischer S, Garcia KC, Gouaux E
Nat Protoc. 2014 Nov;9(11):2574-85. doi: 10.1038/nprot.2014.173. Epub 2014 Oct 9. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
160451	pEG BacMam	vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein
160680	pEG BacMam N term His8 GFP	vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein
160681	pEG BacMam C term GFP His8	Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein
160682	pEG BacMam N term myc eGFP 3C	Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein
160683	pEG BacMam N term StrepII eGFP 3C	Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein
160684	pEG BacMam N term His8 eGFP 3C	Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein
160685	pEG BacMam C term 3C eGFP myc	Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein
160686	pEG BacMam C term 3C eGFP StrepII	Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein
160687	pEG BacMam C term 3C eGFP His8	Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein