Synthetic recombinase-based state machines in living cells.

Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
Science. 2016 Jul 22;353(6297):aad8559. doi: 10.1126/science.aad8559. Epub 2016 Jul 21. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
79540	pNR64	Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.
79541	pNR220	Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.
79542	pNR160	Output plasmid: contains 5-state register made up of TP901 and BxbI recognition sites.
79543	pNR188	Output plasmid: contains 16-state register made up of TP901, BxbI, and A118 recognition sites.
79544	pNR163	Output plasmid: contains 5-state register that regulates expression of GFP.
79545	pNR164	Output plasmid: contains 5-state register that regulates expression of GFP.
79546	pNR230	BxbI attB-attP. GFP will express if sites recombine.
79547	pNR166	Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.
79548	pNR292	Output plasmid: contains 16-state register that regulates expression of GFP. Implements RSM with 3-input passcode behavior.
79550	pNR284	Output plasmid: contains 16-state register that regulates expression of GFP.
79552	pNR186	Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.
79553	pNR187	Output plasmid: contains 5-state register that regulates expression of GFP.
79555	pNR291	Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.
79576	pNR239	A118 attB-attP. GFP will express if sites recombine.
79577	pNR276	TP901 attB-attP. GFP will express if sites recombine.
79578	pNR279	BxbI attL-attR. GFP expression will stop if sites recombine.
79579	pNR280	A118 attL-attR. GFP expression will stop if sites recombine.
79580	pNR287	TP901 attL-attR. GFP expression will stop if sites recombine.
79646	pNR165	Output plasmid: contains 5-state register that regulates expression of GFP.