Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library.
Zhu S, Li W, Liu J, Chen CH, Liao Q, Xu P, Xu H, Xiao T, Cao Z, Peng J, Yuan P, Brown M, Liu XS, Wei W
Nat Biotechnol. 2016 Dec;34(12):1279-1286. doi: 10.1038/nbt.3715. Epub 2016 Oct 31.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89638 | pLenti-sgRNA-lib 2.0 | sgRNA expression in mammalian cells after Gateway cloning |
| 89639 | scaffold-linker-U6 | construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter. |
| 89640 | Wei lab paired-guide RNA (pgRNA) CRISPR–Cas9 library for human long non-coding RNAs (lncRNAs) | Paired sgRNA library containing 12,472 pgRNAs targeting 671 human long non-coding RNAs (lncRNAs) |