Mouse Antibody of IgM Class is Prone to Non-Enzymatic Cleavage between CH1 and CH2 Domains.
Klaus T, Stalinska K, Czaplicki D, Mak P, Skupien-Rabian B, Kedracka-Krok S, Wiatrowska K, Bzowska M, Machula M, Bereta J
Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1038/s41598-017-19003-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 91729 | pFUSEss-CHIg-mM_M18 | Expression plasmid coding for heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. |
| 91730 | pFUSEss-CHIg-mM-His204Ala_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: His204Ala (EU numbering). |
| 91731 | pFUSEss-CHIg-mM-Tyr205Ala_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Tyr205Ala (EU numbering). |
| 91732 | pFUSEss-CHIg-mM-Lys208Ala_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Lys208Ala (EU numbering). |
| 91733 | pFUSEss-CHIg-mM-Asn209Ala_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering). |
| 91734 | pFUSEss-CHIg-mM-Arg210Ala_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg210Ala (EU numbering). |
| 91735 | pFUSEss-CHIg-mM-Asp212Ala_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asp212Ala (EU numbering). |
| 91736 | pFUSEss-CHIg-mM-Leu213Ala_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Leu213Ala (EU numbering). |
| 91737 | pFUSEss-CHIg-mM-His214Ala_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: His214Ala (EU numbering). |
| 91738 | pFUSEss-chimeric-IgM-mouse/human_M18 | Expression plasmid coding for modified heavy chain of mouse IgM antibody. Residues 203-239 (EU numbering) were exchanged to human homologue sequence. |
| 91739 | pFUSEss-CHIg-mM-Asp212Ser_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asp212Ser (EU numbering) - N-glycosylated Asn209 |
| 91740 | pFUSEss-CHIg-mM-Asn209Arg_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Arg (EU numbering). |
| 91741 | pFUSEss-CHIg-mM-Asn209Cys_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Cys (EU numbering). |
| 91742 | pFUSEss-CHIg-mM-Asn209Gln_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Gln (EU numbering). |
| 91743 | pFUSEss-CHIg-mM-Asn209Glu_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Glu (EU numbering). |
| 91744 | pFUSEss-CHIg-mM-Asn209Gly_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Gly (EU numbering). |
| 91745 | pFUSEss-CHIg-mM-Asn209His_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209His (EU numbering). |
| 91746 | pFUSEss-CHIg-mM-Asn209Ile_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ile (EU numbering). |
| 91747 | pFUSEss-CHIg-mM-Asn209Leu_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Leu (EU numbering). |
| 91748 | pFUSEss-CHIg-mM-Asn209Lys_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Lys (EU numbering). |
| 91749 | pFUSEss-CHIg-mM-Asn209Met_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Met (EU numbering). |
| 91750 | pFUSEss-CHIg-mM-Asn209Phe_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Phe (EU numbering). |
| 91751 | pFUSEss-CHIg-mM-Asn209Pro_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Pro (EU numbering). |
| 91752 | pFUSEss-CHIg-mM-Asn209Ser_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ser (EU numbering). |
| 91753 | pFUSEss-CHIg-mM-Asn209Thr_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Thr (EU numbering). |
| 91754 | pFUSEss-CHIg-mM-Asn209Trp_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Trp (EU numbering). |
| 91755 | pFUSEss-CHIg-mM-Asn209Tyr_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Tyr (EU numbering). |
| 91756 | pFUSEss-CHIg-mM-Asn209Val_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Val (EU numbering). |
| 91781 | pFUSEss-CHIg-mM-Asn209Asp_M18 | Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Asp (EU numbering). |