Biosensor libraries harness large classes of binding domains for construction of allosteric transcriptional regulators.
Juarez JF, Lecube-Azpeitia B, Brown SL, Johnston CD, Church GM
Nat Commun. 2018 Aug 6;9(1):3101. doi: 10.1038/s41467-018-05525-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102922 | pHC_DYOLacI-R | pUC-19 derivative reporter plasmid for the screening of Transcription Factors (TFs) carrying the DNA-binding domain of LacI |
| 102924 | pCKTRBS-OD | pCK01 derivative carrying tetR under a constitutive promoter and an aTc-inducible PtetO promoter for control expression of genes. Contains LacI OD for translational fusions on the Ct of cloned genes. |
| 102925 | pCKTRBS-CbnR-ABE44898-OD | pCKTRBS-OD derivative carrying CbnR-ABE44898-OD chimera, also known as ChBz01 |
| 102926 | pCKTRBS-LmrR-BzdB1_nSP | pCKTRBS derivative carrying LmrR-BzdB1_nSP chimera, also known as ChBz02 |