Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling.
Uda Y, Goto Y, Oda S, Kohchi T, Matsuda M, Aoki K
Proc Natl Acad Sci U S A. 2017 Oct 24. pii: 201707190. doi: 10.1073/pnas.1707190114.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 100280 | pCAGGS-PHFF | Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells. |
| 100281 | pCAGGS-hPhyB-mCherry-HRasCT | Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells |
| 100282 | pCAGGS-hPhyB621-mCherry-HRasCT | Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells |
| 100283 | pCAGGS-PIF3-mEGFP | Expresses PIF3 (1-100 aa) fused with mEGFP in mammalian cells |
| 100285 | pCAGGS-PHFF-sh-hBVRA | Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells |
| 100286 | pCAGGS-PHFF-sh-mBVRA | Expression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells |
| 100540 | pCAGGS-hPhyBY276H-mCherry-HRasCT | Expresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells |