DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.
Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, Tuschl T
Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. Epub 2017 Mar 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 70058 | pLKO.1-shDnd1-No1 | TRC lentiviral vector for shRNA against mouse Dnd1 #1 |
| 70060 | pLKO.1-shDnd1-No3 | TRC lentiviral vector for shRNA against mouse Dnd1 #3 |
| 70065 | pLKO.1-shDnd1-No4 | TRC lentiviral vector for shRNA against mouse Dnd1 #4 |
| 70066 | pLKO.1-shDnd1-No5 | TRC lentiviral vector for shRNA against mouse Dnd1 #5 |
| 70067 | pLKO.1-shDnd1-No6 | TRC lentiviral vector for shRNA against mouse Dnd1 #6 |
| 70068 | pLKO.1-shDnd1-No7 | TRC lentiviral vector for shRNA against mouse Dnd1 #7 |
| 70069 | pLKO.1-shDnd1-No8 | TRC lentiviral vector for shRNA against mouse Dnd1 #8 |
| 70070 | pENTR4-hDND1 | Gateway entry vector for human DND1 with stop codon |
| 70071 | pLVE-FH-IN | 2nd gen lentiviral vector. Cre-removable and tet/dox controllable expression of FLAG-HA-tagged transgene in mammalian cells |
| 70072 | pLVE-FH-Dnd1-IN | 2nd gen lentiviral vector. Cre-removable and tet/dox-controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells. |
| 70075 | pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G | 2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells |
| 70076 | pCS-TRE-FHDnd1-Ubc-rtTA-I2G | 2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells |
| 70082 | pET11d-FHis-DND1-Y102A | Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression. |
| 70083 | pET11d-FHis-DND1 | Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein. Codons are optimized for bacteria expression. |
| 106333 | pBRPB CAG-mCherry-IP | PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells. |
| 106334 | pENTR4-EGFP_opt | Gateway Entry vector for EGFP (codon optimized for efficient gene synthesis) |
| 106343 | pLVCT-Dnd1-EGFP | Lentiviral vector, mammalian expression of Dnd1-EGFP fusion protein |
| 106345 | PX459 mDnd1#1 | small guide RNA #1 against RRM-coding region of Dnd1 gene locus |
| 106346 | PX459 mDnd1#2 | small guide RNA #2 against RRM-coding region of Dnd1 gene locus |
| 106347 | PX459 mDnd1#3 | small guide RNA #3 against RRM-coding region of Dnd1 gene locus |
| 106348 | pFRT-TO-DEST | Destination vector for gateway cloning. Developed for Flp-In T-REx system. |