The TIA1 RNA-Binding Protein Family Regulates EIF2AK2-Mediated Stress Response and Cell Cycle Progression.
Meyer C, Garzia A, Mazzola M, Gerstberger S, Molina H, Tuschl T
Mol Cell. 2018 Feb 15;69(4):622-635.e6. doi: 10.1016/j.molcel.2018.01.011.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 106089 | pENTR4_TIAL1 | Gateway entry vector containing TIAL1 |
| 106090 | pFRT_TO_FlagHA_TIAL1 | gene expression of FlagHA-TIAL1 |
| 106091 | pFRT_TO_eGFP_TIAL1 | gene expression of EGFP-TIAL1 |
| 106092 | pET28a_TIAL1 | bacterial protein expression of TIA1 |
| 106093 | pFRT_TO_FlagHA_TIA1 | gene expression of FLAGHA-TIA1 |
| 106094 | pFRT_TO_eGFP_TIA1 | gene expression of EGFP-TIA1 |
| 106095 | pET28a_TIA1 | bacterial protein expression of TIA1 |
| 106096 | px458_2A_GFP_sgRNA_TIAL1 | CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIAL1 |
| 106097 | px458_2A_GFP_sgRNA_TIA1 | CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIA1 |
| 106098 | pENTR4_CELF1 | Entry vector for CELF1 |
| 106099 | pFRT_TO_FlagHA_CELF1 | Inducible gene expression of FlagHA - CELF1 |
| 106100 | pFRT_TO_eGFP_CELF1 | Inducible gene expression of EGFP - CELF1 |
| 106101 | px458_2A_GFP_sgRNA_CELF1 | CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF1 |
| 106102 | px458_2A_GFP_sgRNA_CELF2 | CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF2 |
| 106103 | pENTR4_ELAVL1 | Entry vector for ELAVL1 |
| 106104 | pFRT_TO_FlagHA_ELAVL1 | Inducible gene expression of FlagHA ELAVL1 |
| 106105 | pFRT_TO_eGFP_ELAVL1 | Inducible gene expression of EGFP ELAVL1 |
| 106106 | px458_2A_GFP_sgRNA_ELAVL1 | CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL1 |
| 106107 | px458_2A_GFP_sgRNA_ELAVL2 | CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL2 |
| 106108 | px458_2A_GFP_sgRNA_EIF2AK2 | CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting EIF2AK2 |
| 106109 | px458_2A_GFP_sgRNA_PRKRA | CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting PRKRA |
| 106110 | pENTR4_PRKRA | Entry vector for PRKRA |
| 106111 | pFRT_TO_FlagHA_PRKRA | Inducible gene expression of FlagHA-PRKRA |
| 106112 | pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutant | Inducible gene expression of FlagHA-PRKRA sgRNA PAM mutant |
| 106113 | pLenti_C_Myc_DDK_IRES_Puro_PRKRA | Lentiviral expression of PRKRA |
| 106114 | pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta1 | Lentiviral expression of PRKRA delta1 |
| 106115 | pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta3 | Lentiviral expression of PRKRA delta3 |
| 106116 | pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta12 | Lentiviral expression of PRKRA delta 12 |
| 106117 | pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta23 | Lentiviral expression of PRKRA delta23 |
| 106118 | pLenti_C_Myc_DDK_IRES_Puro_UFD1L | Lentiviral expression of UFD1L |
| 106119 | pLenti_C_Myc_DDK_IRES_Puro_TARBP2 | Lentiviral expression of TARBP2 |
| 106120 | pLenti_C_Myc_DDK_IRES_Puro_ALDH7A1 | Lentiviral expression of ALDH7A1 |