Serine Catabolism by SHMT2 Is Required for Proper Mitochondrial Translation Initiation and Maintenance of Formylmethionyl-tRNAs.

Minton DR, Nam M, McLaughlin DJ, Shin J, Bayraktar EC, Alvarez SW, Sviderskiy VO, Papagiannakopoulos T, Sabatini DM, Birsoy K, Possemato R
Mol Cell. 2018 Feb 15;69(4):610-621.e5. doi: 10.1016/j.molcel.2018.01.024. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
106299	pMXS-IRES-Blast coSHMT2	Expresses SHMT2
106301	pMXS-IRES-Blast SHMT2res	Expresses SHMT2
106302	pMXS-IRES-Blast SHMT2 CD	Expresses SHMT2 K280A catalytic site mutant
106304	pMXS-IRES-Blast SHMT1	Express SHMT1
106305	pSECB	Express spCas9 with sgRNA, blast resistance
106306	pSECB sgMTHFD2L_1	Express Cas9 and sgRNA targeting MTHFD2L
106307	pSECB sgMTHFD2L_2	Express Cas9 and sgRNA targeting MTHFD2L
106308	pLenti CRISPR sgSHMT2_1	Express Cas9 and sgRNA targeting SHMT2
106310	pLenti CRISPR sgSHMT2_2	Express Cas9 and sgRNA targeting SHMT2
106311	pLenti CRISPR sgSHMT1_1	Express Cas9 and sgRNA targeting SHMT1
106312	pLenti CRISPR sgSHMT1_2	Express Cas9 and sgRNA targeting SHMT1
106313	pLenti CRISPR sgMTHFD2_1	Express Cas9 and sgRNA targeting MTHFD2
106314	pLenti CRISPR V2 sgShmt2_1	Express Cas9 and sgRNA targeting mShmt2
106315	pLenti CRISPR V2 sgShmt2_2	Express Cas9 and sgRNA targeting mShmt2
106316	pLenti CRISPR V2 sgMTHFD1L_1	Express Cas9 and sgRNA targeting MTHFD1L
106317	pLenti CRISPR V2 sgMTHFD1L_2	Express Cas9 and sgRNA targeting MTHFD1L
106318	pLenti CRISPR V2 sgMTFMT_1	Express Cas9 and sgRNA targeting MTFMT
106319	pLKO.1P shSHMT2	Express shRNA targeting SHMT2