Deep Mutagenesis of a Transporter for Uptake of a Non-Native Substrate Identifies Conformationally Dynamic Regions
Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
bioRxiv 2021.04.19.440442
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107456 | pCEP4-SERT-myc | Mammalian expression plasmid for codon optimized hSERT with internal MYC tag inserted in ECL2 |
| 107457 | pCEP4-DAT-myc | Mammalian expression plasmid for codon optimized hDAT with internal MYC tag inserted in ECL2 |
| 107458 | pCEP4-NET-myc | Mammalian expression plasmid for codon optimized hNET with internal MYC tag inserted in ECL2 |
| 107459 | pCEP4-SERTG56A -myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G56A conferring wildtype phenotype |
| 107460 | pCEP4-SERTA169D-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A169D conferring gain of function phenotype, decreased surface expression |
| 107461 | pCEP4-SERTA331T-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A331T conferring gain of function phenotype |
| 107462 | pCEP4-SERTS375L-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S375L with lower surface expression relative to wildtype |
| 107463 | pCEP4-SERTG384S-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G384S with loss of surface expression relative to wildtype |
| 107464 | pCEP4-SERTI425V-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I425V conferring aproximately wildtype phenotype |
| 107465 | pCEP4-SERTI425L-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I425L conferring approximately wildtype transport phenotype, decreased surface expression |
| 107466 | pCEP4-SERTT439M-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T439M, decreased surface expression and transport relative to wildtype |
| 107467 | pCEP4-SERTT448K-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T448K conferring gain of function phenotype, decreased surface expression |
| 107468 | pCEP4-SERTR464Q-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R464Q approximately wildtype transport ability |
| 107469 | pCEP4-SERTF465L-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F465L conferring gain of function phenotype |
| 107470 | pCEP4-SERTS477F-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S477F loss of transport relative to wildtype, decreased surface expression |
| 107471 | pCEP4-SERTG484E-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G484E decreased transport and surface expression relative to wildtype |
| 107472 | pCEP4-SERTL550V-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L550V approximately wildtype transport |
| 107473 | pCEP4-SERTI576V-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I576V approximately wildtype transport |
| 107474 | pCEP4-SERTN177E-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation N177E with approximately wildtype activity |
| 107475 | pCEP4-SERTK605N-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K605N approxmately wildtype activity, decreased surface expression |
| 107476 | pCEP4-SERTK37Q-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K37Q approximately wildtype activity |
| 107477 | pCEP4-SERTR79V-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R79V conferring gain of function phenotype, decreased surface expression |
| 107478 | pCEP4-SERTF88H-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F88H conferring gain of function phenotype |
| 107479 | pCEP4-SERTA173G-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A173G with approximately wildtype activity, decreased surface expression |
| 107480 | pCEP4-SERTY289I-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation Y289I with approximately wildtype activity |
| 107481 | pCEP4-SERTT233F-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T233F with approximately wildtype activity |
| 107482 | pCEP4-SERTF263C-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F263C with approximately wildtype activity, decreased surface expression |
| 107483 | pCEP4-SERTR596Q-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R596Q conferring gain of function phenotype |
| 107484 | pCEP4-SERTV343T-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation V343T conffering gain of function phenotype, decreased surface expression |
| 107485 | pCEP4-SERTI379Q-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I379Q conferring gain of function phenotype, decreased surface expression |
| 107486 | pCEP4-SERTE392M-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation E392M with approximately wildtype activity |
| 107487 | pCEP4-SERTL406I-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L406I with approximately wildtype activity |
| 107488 | pCEP4-SERTY410G-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation Y410G with approximately wildtype activity, decreased surface expression |
| 107489 | pCEP4-SERTR462E-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R462E with approximately wildtype activity, decreased surface expression |
| 107490 | pCEP4-SERTL467D-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L467D with approximately wildtype activity, decreased surface expression |
| 107491 | pCEP4-SERTC473H-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation C473H conferring gain of function phenotype |
| 107492 | pCEP4-SERTK490L-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K490L conferring gain of function phenotype, decreased surface expression |
| 107493 | pCEP4-SERTE494Y-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation E494Y conferring gain of function phenotype |
| 107494 | pCEP4-SERTP499K-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation P499K conferring gain of function phenotype |
| 107495 | pCEP4-SERTF556H-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F556H conferring gain of function phenotype |
| 107496 | pCEP4-SERTI576K-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I576K with approximately wildtype activity, decreased surface expression |
| 107497 | pCEP4-SERTG582K-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G582K conferring gain of function phenotype, decreased surface expression |
| 107498 | pCEP4-SERTS585P-myc | Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S585P conferring gain of function phenotype |