protaTETHER - a method for the incorporation of variable linkers in protein fusions reveals impacts of linker flexibility in a PKAc-GFP fusion protein.
Norris JL, Hughes RM
FEBS Open Bio. 2018 Apr 25;8(6):1029-1042. doi: 10.1002/2211-5463.12414. eCollection 2018 Jun.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108579 | PKAcs-nolinker-GFP | Expresses PKAcs-GFP fusion in mammalian cells |
| 108583 | PKAcs-GGS4X-GFP | Expresses PKAcs-GFP fusion in mammalian cells with flexible linker |
| 108584 | PKAcs-GGSD5X-GFP | Expresses PKAcs-GFP fusion in mammalian cells with flexible linker |
| 108585 | PKAcs-KR6X-GFP | Expresses PKAcs-GFP fusion in mammalian cells with structured linker |
| 108587 | PKAcs-L10X-GFP | Expresses PKAcs-GFP fusion in mammalian cells with structured linker |
| 108588 | PKAcs-G12X-GFP | Expresses PKAcs-GFP fusion in mammalian cells with flexible linker |