Transgene-free genome editing in marine algae by bacterial conjugation - comparison with biolistic CRISPR/Cas9 transformation.
Sharma AK, Nymark M, Sparstad T, Bones AM, Winge P
Sci Rep. 2018 Sep 26;8(1):14401. doi: 10.1038/s41598-018-32342-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 109219 | PtPuc3_diaCas9_sgRNA | Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum |