Genetically encoded multimode reporter of AP-3 traffic in budding yeast.
Plemel RL, Odorizzi G, Merz AJ
Traffic. 2020 Nov 23. doi: 10.1111/tra.12772.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 111450 | pCYC1pr-GNSI (LEU) | GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts. |
| 121537 | pSUC2::GNSI (NrsR) | This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus. |