In-Frame and Unmarked Gene Deletions in Burkholderia cenocepacia via an Allelic Exchange System Compatible with Gateway Technology.
Fazli M, Harrison JJ, Gambino M, Givskov M, Tolker-Nielsen T
Appl Environ Microbiol. 2015 Jun;81(11):3623-30. doi: 10.1128/AEM.03909-14. Epub 2015 Mar 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 111608 | pDONRPEX18Tp-SceI-pheS | Gateway compatible allelic exchange vector for Burkholderia sp. |
| 111609 | pDONRPEX18Gm-SceI-pheS | Gateway compatible allelic exchange vector for Burkholderia sp. |
| 111610 | pDONRPEX18Km-SceI-pheS | Gateway compatible allelic exchange vector for Burkholderia sp. |
| 111611 | pDAI-SceI-pheS | Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp. |