Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation.
Liu LD, Huang M, Dai P, Liu T, Fan S, Cheng X, Zhao Y, Yeap LS, Meng FL
Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112127 | pLL1 sgRNA(MS2)-MS2-AIDmono | empty sgRNA cloning vector with MS2-AIDmono |
| 112128 | pLL2 sgRNA(MS2)-MS2-AID3c | empty sgRNA cloning vector with MS2-AID3c |
| 112129 | pLL3 sgRNA(MS2)-MS2-AID3f | empty sgRNA cloning vector with MS2-AID3f |
| 112130 | pLL4 sgRNA(MS2)-MS2-hA3a | empty sgRNA cloning vector with MS2-humanA3a |
| 112131 | pLL5 sgRNA(MS2)-MS2-hA3a D131A | empty sgRNA cloning vector with MS2-humanA3a D131A |
| 112132 | pLL6 sgRNA(MS2)-MS2-hA3a D131E | empty sgRNA cloning vector with MS2-humanA3a D131E |
| 112133 | pLL7 lenti-dcas9-3xflag-blast | lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE) |
| 112134 | pLL8 lenti-D10Acas9-3xflag-blast | lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE) |