Marko Hyvönen Lab: Protein expressionists toolkit

Lulla A, Hyvönen M
unpublished

Plasmids from Article

ID	Plasmid	Purpose
112558	pExp-NHis	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag
112559	pExp-CHis	Used for expression of protein in E. coli as C-terminual 8His fusions
112560	pExp-AFV	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and AFV (Acidianus Filamentous Virus 1 protein) fusions with optional C-terminal StrepII-tag
112561	pExp-Bla	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tag
112562	pExp-DsbA	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag
112563	pExp-DsbA(SS)	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag
112564	pExp-DsbC	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tag
112565	pExp-GB1	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag
112566	pExp-GST	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST fusions with optional C-terminal StrepII-tag
112567	pExp-IF2	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and IF2 (domain I of translation initiation factor IF-2 from E. coli) fusions with optional C-terminal StrepII-tag
112568	pExp-MBP	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag
112569	pExp-mCherry	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag
112570	pExp-MsyB	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tag
112571	pExp-NusA	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tag
112572	pExp-PpiB	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag
112573	pExp-sfGFP	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag
112574	pExp-Sumo	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag
112575	pExp-TF	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tag
112576	pExp-TFSumo	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli linked to Sumo) fusions with optional C-terminal StrepII-tag
112577	pExp-Trx	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag
112578	pExp-Tsf	Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Tsf (elongation factor Ts from E. coli fusions with optional C-terminal StrepII-tag
129237	pExp-Lipo	Protein expression in E.coli
129238	pExp-Calm	Protein expression in E.coli
129239	pExp-LSL	Protein expression in E.coli
129240	pExp-Spy	Protein expression in E.coli
129241	pExp-Skp	Protein expression in E.coli
129243	pExp-DsbC(SS)	Protein expression in E.coli
129245	pExp-Stag	Protein expression in E.coli
129246	pExp-T7tag	Protein expression in E.coli
129247	pExp-Etag	Protein expression in E.coli