The Atg17-Atg31-Atg29 Complex Coordinates with Atg11 to Recruit the Vam7 SNARE and Mediate Autophagosome-Vacuole Fusion.
Liu X, Mao K, Yu AYH, Omairi-Nasser A, Austin J 2nd, Glick BS, Yip CK, Klionsky DJ
Curr Biol. 2016 Jan 25;26(2):150-160. doi: 10.1016/j.cub.2015.11.054. Epub 2016 Jan 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 121386 | pGAD-Atg17 | For Yeast-two-hybrid analysis |
| 121387 | pGAD-Atg17(I98E) | For Yeast-two-hybrid analysis |
| 121388 | pGAD-Atg17(L105D) | For Yeast-two-hybrid analysis |
| 121389 | pGAD-Atg17(I310D) | For Yeast-two-hybrid analysis |
| 121390 | pGAD-Atg17(L313D I314K) | For Yeast-two-hybrid analysis |
| 121391 | pGAD-Atg17(F317D) | For Yeast-two-hybrid analysis |
| 121392 | pGAD-Atg17(I325D) | For Yeast-two-hybrid analysis |
| 121393 | pGAD-Atg17(L331D) | For Yeast-two-hybrid analysis |
| 121394 | pGBDU-Atg17 | For Yeast-two-hybrid analysis |
| 121395 | pGBDU-Atg17(L105D) | For Yeast-two-hybrid analysis |
| 121396 | pGBDU-Atg17(F317D) | For Yeast-two-hybrid analysis |
| 121397 | pGBDU-Atg17(I325D) | For Yeast-two-hybrid analysis |
| 121405 | pCu-Atg17(416) (L105D) | Expressing Atg17 |
| 121406 | pCu-Atg17(416) (F317D) | Expressing Atg17 |
| 121407 | pCu-Atg17(416) (I325D) | Expressing Atg17 |
| 121408 | pCu-GFP-Atg17(416) | Expressing GFP-Atg17 |
| 121409 | pCu-GFP-Atg17(416) (L313D I314K) | Expressing GFP-Atg17 |
| 121410 | pCu-GFP-Atg17(416) (L105D) | Expressing GFP-Atg17 |
| 121411 | pCu-GFP-Atg17(416) (F317D) | Expressing GFP-Atg17 |
| 121412 | pCu-GFP-Atg17(416) (I325D) | Expressing GFP-Atg17 |
| 121515 | pGAD-Atg17(∆15-106) | For Yeast-two-hybrid analysis |
| 121516 | pGAD-Atg17(∆77-91) | For Yeast-two-hybrid analysis |
| 121517 | pGAD-Atg17(∆92-106) | For Yeast-two-hybrid analysis |
| 121518 | pGAD-Atg17(∆129-177) | For Yeast-two-hybrid analysis |
| 121519 | pGAD-Atg17(∆186-236) | For Yeast-two-hybrid analysis |
| 121520 | pGAD-Atg17(∆266-280) | For Yeast-two-hybrid analysis |
| 121521 | pGAD-Atg17(∆266-312) | For Yeast-two-hybrid analysis |
| 121522 | pGAD-Atg17(∆279-295) | For Yeast-two-hybrid analysis |
| 121523 | pGAD-Atg17(∆296-312) | For Yeast-two-hybrid analysis |
| 121524 | pGAD-Atg17(∆313-334) | For Yeast-two-hybrid analysis |
| 121525 | pGAD-Atg17(∆335-417) | For Yeast-two-hybrid analysis |
| 122268 | pGAD-Atg17(F317G) | For Yeast-two-hybrid analysis |
| 122269 | pGAD-Atg17(F317H) | For Yeast-two-hybrid analysis |
| 122270 | pGAD-Atg17(F317K) | For Yeast-two-hybrid analysis |
| 122271 | pGAD-Atg17(F317Q) | For Yeast-two-hybrid analysis |
| 122272 | pGAD-Atg17(I325A) | For Yeast-two-hybrid analysis |
| 122273 | pGAD-Atg17(F317D, I325A) | For Yeast-two-hybrid analysis |
| 122274 | pGBDU-Atg17(I325A) | For Yeast-two-hybrid analysis |
| 122275 | pGBDU-Atg17(F317D, I325A) | For Yeast-two-hybrid analysis |