Nuclear actin interactome analysis links actin to KAT14 histone acetyl transferase and mRNA splicing.
Viita T, Kyheroinen S, Prajapati B, Virtanen J, Frilander MJ, Varjosalo M, Vartiainen MK
J Cell Sci. 2019 Apr 17;132(8). pii: jcs.226852. doi: 10.1242/jcs.226852.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118366 | Flag-GFP11-pDEST-N | Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners. |
| 118367 | Flag-GFP11-pDEST-C | Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners. |
| 118368 | HA-GFP1-10-pDEST-N | Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners. |
| 118369 | HA-GFP1-10-pDEST-C | Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners. |
| 118370 | HA-GFP1-10-actin-pcDNA4-TO | Bimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin. |
| 118371 | 2Flag-pDEST-N | Gateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest. |
| 118372 | 2Flag-pDEST-C | Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest. |
| 118373 | 2HA-pDEST-N | Gateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest. |
| 118374 | 2HA-pDEST-C | Gateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest. |
| 118375 | HA-BirA*-pDEST-N-pcDNA5/FRT/TO | Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest. |
| 118376 | pENTR-KAT14-547-782 | Gateway compatible donor vector with KAT14-547-782 truncation. |
| 118377 | pENTR-KAT14-1-546 | Gateway compatible donor vector with KAT14-1-546 truncation. |
| 118378 | pENTR-actin | Gateway compatible donor vector with WT actin. |
| 118379 | pENTR-actin-R62D | Gateway compatible donor vector with actin-R62D. |
| 118380 | pENTR-NLS-actin | Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin. |
| 118381 | pENTR-NLS-actin-R62D | Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D. |
| 118382 | KAT14-pDEST10 | HIS-tagged insect cell expression vector with KAT14. |
| 118383 | pENTR-actin-V159N | Gateway compatible donor vector with actin-V159N. |
| 118384 | pENTR-actin-G168D,Y169D | Gateway compatible donor vector with actin-G168D,Y169D. |