Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites.

Liu Y, Cao Z, Wang Y, Guo Y, Xu P, Yuan P, Liu Z, He Y, Wei W
Nat Biotechnol. 2018 Nov 5. pii: nbt.4283. doi: 10.1038/nbt.4283. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
119976	pLenti-sgRNA(lib)-puro	Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
119977	Splicing-targeting CRISPR-Cas9 library for human lncRNAs	Designed for genomic deletion screening of functional lncRNAs by targeting their splice sites.