The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, Khavari PA
Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 10.1016/j.molcel.2018.12.001.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120558 | pLEX-uORF-HA-birA*-STOP-IRES-Puro | Expresses control HA-birA* in mammalian cells & for virus production |
| 120559 | pLEX-uORF-HA-birA*-H-Ras(wildtype)-IRES-Puro | Expresses HA-birA*-H-Ras wildtype fusion protein in mammalian cells & for virus production |
| 120560 | pLEX-uORF-HA-birA*-H-Ras(G12V)-IRES-Puro | Expresses HA-birA*-H-Ras G12V mutant fusion protein in mammalian cells & for virus production |
| 120561 | pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro | Expresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells & for virus production |
| 120562 | pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro | Expresses HA-birA*-K-Ras G12D fusion protein in mammalian cells & for virus production |
| 120563 | pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro | Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production |
| 120564 | pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro | Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production |
| 120565 | pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro | Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production |
| 120566 | pLEX-uORF-HA-birA*-N-Ras(Q61K/E37A)-IRES-Puro | Expresses HA-birA*-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production |
| 120567 | pLEX-uORF-HA-birA*-N-Ras(Q61K/Y40A)-IRES-Puro | Expresses HA-birA*-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production |
| 120568 | pLEX-FHH-Empty Vector-IRES-Puro | For cloning genes with an N-terminal TAP tag [Flag-HA-6xHIS] |
| 120569 | pLEX-FHH-NRAS-IRES-Puro | Expresses Flag-HA-6xHIS-N-Ras wildtype fusion protein in mammalian cells & for virus production |
| 120570 | pLEX-FHH-NRASQ61K-IRES-Puro | Expresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian cells & for virus production |
| 120571 | pLEX-FHH-NRASQ61K/T35A-IRES-Puro | Expresses Flag-HA-6xHIS-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production |
| 120572 | pLEX-FHH-NRASQ61K/E37A-IRES-Puro | Expresses Flag-HA-6xHIS-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production |
| 120573 | pLEX-FHH-NRASQ61K/Y40A-IRES-Puro | Expresses Flag-HA-6xHIS-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production |
| 120574 | pLEX-FLAG-NRAS-IRES-Puro | Expresses Flag-N-Ras wildtype fusion protein in mammalian cells & for virus production |
| 120575 | pLEX-FLAG-NRASQ61K-IRES-Puro | Expresses Flag-N-Ras Q61K fusion protein in mammalian cells & for virus production |
| 120576 | pLEX-FLAG-RHEB-IRES-Puro | Expresses Flag-RHEB fusion protein in mammalian cells & for virus production |
| 120705 | pLEX-MAPKAP1.1-FHH-IRES-Puro | Expresses MAPKAP1.1-Flag-HA-6xHIS fusion protein in mammalian cells & for virus production |
| 120706 | pLEX-MAPKAP1Del-FHH-IRES-Puro | Expresses MAPKAP1 Truncation-Flag-HA-6xHIS fusion protein in mammalian cells & for virus production |
| 120707 | pLEX-FHH-Raf1RBD | Expresses Flag-HA-6xHIS-Raf1 RBD fusion protein in mammalian cells & for virus production |
| 120708 | pLEX-FHH-MTORKinaseDomain | Expresses Flag-HA-6xHIS-Mtor Kinase Domain fusion protein in mammalian cells & for virus production |
| 120709 | pLEX-FHH-MTOR HEAT | Expresses Flag-HA-6xHIS-Mtor HEAT Domain fusion protein in mammalian cells & for virus production |
| 120710 | pLEX-FHH-MAPKAP1RBD | Expresses Flag-HA-6xHIS-MAPKAP1 RBD fusion protein in mammalian cells & for virus production |
| 120711 | pLEX-FHH-MAPKAP1RBD/Triple Mutant | Expresses Flag-HA-6xHIS-MAPKAP1 RBD Triple Mutant fusion protein in mammalian cells & for virus production |
| 120713 | pLEX-Frb:Akt2-IRES-Hygromycin | Expresses Frb:Akt2 fusion protein in mammalian cells & for virus production |
| 120714 | pLEX-FKBP-Kras-Blasticidin | Expresses membrane-targeted recruiter protein in mammalian cells & for virus production |
| 120715 | pLEX-FKBP-Rab5-Blasticidin | Expresses early endosome-targeted recruiter protein in mammalian cells & for virus production |
| 120716 | pLEX-FKBP-Rab7a-Blasticidin | Expresses late endosome-targeted recruiter protein in mammalian cells & for virus production |
| 120717 | pLEX-FKBP-Rab11-Blasticidin | Expresses recycling endosome-targeted recruiter protein in mammalian cells & for virus production |
| 120718 | pLEX-FKBP-BclXL-Blasticidin | Expresses mitochondrial membrane-targeted recruiter protein in mammalian cells & for virus production |
| 120723 | pLEX-TCRb-FKBP-Blasticidin | Expresses ER-targeted recruiter protein in mammalian cells & for virus production |
| 120798 | pRRL Luciferase | For production of lentivirus to express luciferase, primarily for in vivo bioluminescence tracking experiments. |