Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins.
Mangeot PE, Risson V, Fusil F, Marnef A, Laurent E, Blin J, Mournetas V, Massourides E, Sohier TJM, Corbin A, Aube F, Teixeira M, Pinset C, Schaeffer L, Legube G, Cosset FL, Verhoeyen E, Ohlmann T, Ricci EP
Nat Commun. 2019 Jan 3;10(1):45. doi: 10.1038/s41467-018-07845-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 119942 | BIC-Gag-CAS9 | encodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes. |
| 119971 | GAG-CRErec | Expresses GAG (FMLV) fused with CRE recombinase for the production of VLPs loaded with CRE protein |
| 120922 | BICstim-Gag-dCAS9-VPR | encodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation. |
| 134912 | BLADE | Empty backbone for cloning sgRNA sequence to be used in nanoblades system |
| 134913 | SUPERBLADE5 | Empty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013) |
| 134914 | BLADE-182 | sgRNA targeting GFP to be used in nanoblade system |