Pooled library screening with multiplexed Cpf1 library.

Liu J, Srinivasan S, Li CY, Ho IL, Rose J, Shaheen M, Wang G, Yao W, Deem A, Bristow C, Hart T, Draetta G
Nat Commun. 2019 Jul 17;10(1):3144. doi: 10.1038/s41467-019-10963-x. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
127749	LentiUniversal-Puro	Lentiviral plasmid for expressing any U6 driven transcripts (sgRNAs, crRNAs, shRNAs and etc...)
127750	LentiAsCpf1-3xMYC-blast	Lentiviral plasmid for expressing human codon-optimized AsCpf1 containing 3x N-terminal MYC-NLS signal
130630	Mini-human AsCpf1-based human genome-wide knockout library	Knockout library targeting 16,977 genes. Each gene is targeted by an AsCpf1(AsCas12a)-based array containing 3-4 guides concatenated in one vector.