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Directed Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning.
Unger EK, Keller JP, Altermatt M, Liang R, Matsui A, Dong C, Hon OJ, Yao Z, Sun J, Banala S, Flanigan ME, Jaffe DA, Hartanto S, Carlen J, Mizuno GO, Borden PM, Shivange AV, Cameron LP, Sinning S, Underhill SM, Olson DE, Amara SG, Temple Lang D, Rudnick G, Marvin JS, Lavis LD, Lester HA, Alvarez VA, Fisher AJ, Prescher JA, Kash TL, Yarov-Yarovoy V, Gradinaru V, Looger LL, Tian L
Cell. 2020 Dec 12. pii: S0092-8674(20)31612-3. doi: 10.1016/j.cell.2020.11.040.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
128483pRSET-iSeroSnFRFluorescent reporter for serotonin (bacterial expression)
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128484pDisplay-iSeroSnFRFluorescent reporter for serotonin (mammalian expression, membrane localization tag)
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128485pAAV-CAG-iSeroSnFR-NlgnFluorescent reporter for serotonin (dendrite localization tag)
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128486pAAV-CAG-FLEx-iSeroSnFRFluorescent reporter for serotonin (conditional + membrane localization tag)
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129180pAAV-CAG-FLEx-iSeroSnFR-EnhancedExportFluorescent reporter for serotonin imaging (conditional + membrane localization tag with enhanced export sequence)
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