The oncogenic function of PLAGL2 is mediated via ASCL2 and IGF2 and a Wnt-independent mechanism in colorectal cancer.

Fischer AD, Veronese-Paniagua DA, Swaminathan S, Kashima H, Rubin DC, Madison BB
Am J Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 10.1152/ajpgi.00058.2022. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
133355	BII-gR-PnGW	Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications), co-expressed with GFP-NLS
133357	BII-gR-PtW-eSpCas9	Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression
133358	BII-gR-BtW-eSpCas9	Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression
133359	BII-Sh-PnGW	Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS
133360	BII-Sh-PnGW-Luc	Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS
133361	BII-Sh-PIR702W	Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS
133362	BII-Sh-PIR702W-Luc	Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS
133363	BII-Sh-FUCCI	Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter
133364	BII-Sh-FUCCI-Luc	Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter
133366	BII-Sh-PnGW-CMV	Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS
133367	BII-Sh-PnGW-CMV-Luc	Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS
133368	BII-ChBtW	Piggybac vector for constitutive expression
133369	BII-ChBtW-PLAGL2	Piggybac vector for constitutive expression
133370	BII-ChBtW-eSpCas9	Piggybac vector for constitutive expression
133371	BII-ChBtW-iresFUCCI	Piggybac vector for constitutive expression
133372	BII-CBtW	Piggybac vector for constitutive expression
133374	BII-ChBtW-IGF2	Piggybac vector for constitutive expression
133375	BII-ChBtW-ASCL2	Piggybac vector for constitutive expression
133376	BII-ChBtW-CTNNB1_S33Y	Piggybac vector for constitutive expression
133377	BII-ChPtinGW	Piggybac vector for constitutive expression
133378	BII-ChBtW-dnTCF4-ires-GFP	Piggybac vector for constitutive expression
133379	BII-ChPtW	Piggybac vector for constitutive expression
133380	BII-ChPtW-PLAGL2	Piggybac vector for constitutive expression
133381	BII-ChPtW-eSpCas9	Piggybac vector for constitutive expression
133382	BII-ChiNW	Piggybac vector for constitutive expression
133383	BII-ChiNW-IGF2	Piggybac vector for constitutive expression
133384	BII-ChiNW-ASCL2	Piggybac vector for constitutive expression
133402	BII-CMV-AfmW3A	Piggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cells