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Dimerisation of APOBEC1 is dispensable for its RNA editing activity
Chieca M, Montini M, Severi F, Pecori R, Conticello S
bioRxiv 2021 Article

Plasmids from Article

ID Plasmid Purpose
112860human ACFplasmid to overexpress human APOBEC1-complementation factor (ACF; A1CF) in mammalian cells
112861rat APOBEC1 E63Aplasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells
134606pBlueScript-humanAPOBEC1Plasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activity
134607pEGFP-N1-Flag-humanRBM47Plasmid bearing RBM47 coding sequence. Flag tagged is useful for IP and WB assays.
167169pEGFP ratAPOBEC1 L173A/G227AExpresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.
167171pEGFP humanAPOBEC1 L173A/G227AExpresses human APOBEC1 L173A/G227A in mammalian cells

Antibodies from Article