RNA isoform screens uncover the essentiality and tumor-suppressor activity of ultraconserved poison exons.
Thomas JD, Polaski JT, Feng Q, De Neef EJ, Hoppe ER, McSharry MV, Pangallo J, Gabel AM, Belleville AE, Watson J, Nkinsi NT, Berger AH, Bradley RK
Nat Genet. 2020 Jan;52(1):84-94. doi: 10.1038/s41588-019-0555-z. Epub 2020 Jan 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 138084 | Human CRISPR Poison Exon Knockout Library | Targets 3' splice sites with paired gRNAs. Induces skipping of poison cassette exons and matching upstream constitutive exons. Compare loss of a constitutive coding exon with loss of a poison exon. |