The E3 Ubiquitin Ligase Mind Bomb 1 Controls Adenovirus Genome Release at the Nuclear Pore Complex.
Bauer M, Flatt JW, Seiler D, Cardel B, Emmenlauer M, Boucke K, Suomalainen M, Hemmi S, Greber UF
Cell Rep. 2019 Dec 17;29(12):3785-3795.e8. doi: 10.1016/j.celrep.2019.11.064.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140237 | Lenti-eCas9 | Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold |
| 140238 | Lenti-eCas9-sgNT | Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA |
| 140239 | Lenti-eCas9-sgMIB1 | Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1 |
| 140240 | pLVX-Flag-MIB1-IRES-Puro | Lentiviral vector expressing flag-tagged MIB1 |
| 140241 | pLVX-BSD-tet-mEGFP-MIB1 | Lentiviral vector expressing mEGFP-tagged MIB1 upon tetracycline treatment |