Axonogenesis Is Coordinated by Neuron-Specific Alternative Splicing Programming and Splicing Regulator PTBP2.
Zhang M, Ergin V, Lin L, Stork C, Chen L, Zheng S
Neuron. 2019 Feb 20;101(4):690-706.e10. doi: 10.1016/j.neuron.2019.01.022. Epub 2019 Feb 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 209560 | pEGFP-C1-SHTN1L | Mammalian expression of Shtn1 long isoform |
| 209561 | pEGFP-C1-SHTN1S | Mammalian expression of Shtn1 short isoform |
| 209562 | pEGFP-C1-SHTN1LRRR>GGG | Mammalian expression of Shtn1 long isoform with RG mutation |
| 209563 | pEGFP-C1-deltaExon17 | Mammalian expression of Shtn1 long isoform with E17 deletion |
| 209564 | pEGFP-C1-deltaExon16-17 | Mammalian expression of Shtn1 long isoform with E16-17 deletion |
| 209566 | pEGFP-C1-deltaExon15-II | Mammalian expression of Shtn1 long isoform with E15 deletion II |
| 209567 | pEGFP-C1-deltaExon15-III | Mammalian expression of Shtn1 long isoform with E15 deletion III |
| 209568 | pCAGIG-FLAG-SHTN1L | Mammalian expression of Shtn1 long isoform |
| 209569 | pCAGIG-FLAG-SHTN1S | Mammalian expression of Shtn1 short isoform |
| 209570 | pCAGIG-FLAG-SHTN1LRRR>GGG | Mammalian expression of Shtn1 long isoform with RG mutation |
| 209574 | pFlare9A-Clip2E9 WT | minigene construct for Clip2 E9 alternative splicing |
| 209575 | pFlare9A-Clip2E9 Mutant | minigene construct for Clip2 E9 alternative splicing, potential PTBP2 binding sites are deleted |
| 209576 | pFlare9A-Plekha5E10 WT | minigene construct for Plekha5 E10 alternative splicing |