A molecular network regulating the proinflammatory phenotype of human memory T lymphocytes.
Emming S, Bianchi N, Polletti S, Balestrieri C, Leoni C, Montagner S, Chirichella M, Delaleu N, Natoli G, Monticelli S
Nat Immunol. 2020 Apr;21(4):388-399. doi: 10.1038/s41590-020-0622-8. Epub 2020 Mar 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 149714 | pLVX_BHLHE40-FlagHA_IRES-ZsGreen1 | Lentiviral vector expressing human BHLHE40 with FLAG-HA tags |
| 149718 | pSCALPS_miR-146aSponge_mCherry_GFP | Lentiviral vector expressing a miR-146a decoy sponge |
| 153061 | pLVX-EF1α-BHLHE40-IRES-ZsGreen1 | Lentiviral vector expressing human BHLHE40 |
| 153062 | pLVX-EF1α-BHLHE40 aa1-297-IRES-ZsGreen1 | Lentiviral vector expressing a truncated form of human BHLHE40 (aa 1-297) |
| 153064 | pGL3-Promoter-4xBS_BHLHE40 | Four BHLHE40 consensus binding sites cloned upstream of luciferase reporter gene |
| 153065 | pGL3-Basic-ZC3H12D_PromoterWT | ZC3H12D promoter region cloned upstream of luciferase reporter gene |
| 153066 | pGL3-Enhancer-ZC3H12D_PromoterWT | ZC3H12D promoter region cloned upstream of luciferase reporter gene |
| 153067 | pGL3-Basic-ZC3H12D_PromoterMUT | ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter |
| 153068 | pGL3-Enhancer-ZC3H12D_PromoterMUT | ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter |
| 153069 | pmirGLO-3'UTR-TNF | Human TNF 3'UTR region cloned downstream of luciferase reporter gene |
| 153070 | pmirGLO-3'UTR-IL22 | Human IL-22 3'UTR region cloned downstream of luciferase reporter gene |
| 153071 | pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP | Lentiviral vector expressing human ZC3H12D mutated (D95N) |