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Highly efficient multiplex editing: One-shot generation of 8x Nicotiana benthamiana and 12x Arabidopsis mutants.
Stuttmann J, Barthel K, Martin P, Ordon J, Erickson JL, Herr R, Ferik F, Kretschmer C, Berner T, Keilwagen J, Marillonnet S, Bonas U
Plant J. 2021 Feb 12. doi: 10.1111/tpj.15197.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
153228pDGE347Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
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153229pDGE651Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
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153230pDGE652Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
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153231pDGE666Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
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153232pDGE667Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
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153233pDGE668Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
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153234pDGE463Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
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153235pDGE669Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
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153236pDGE670Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
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153237pDGE671Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
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153238pDGE672Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
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153239pDGE673Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs
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153240pDGE331M1E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153241pDGE332M1 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153242pDGE333M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153243pDGE334M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153244pDGE335M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153245pDGE336M4 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153246pDGE337M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153247pDGE495 M5 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153248pDGE496 M6 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153249pDGE497M6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153250pDGE341 M7 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153251pDGE342M8E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153252pDGE654M1E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153253pDGE655M1 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153254pDGE656M2 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153255pDGE657 M2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153256pDGE658M3 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153257pDGE659 M4 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153258pDGE660M4E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153259pDGE661 M5 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153260pDGE662 M6 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153261pDGE663M6E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153262pDGE664 M7 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153263pDGE665 M8E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153264pDGE473M1E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153265pDGE474 M1 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153266pDGE475 M2 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153267pDGE476 M2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153268pDGE477 M3 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153269pDGE478 M4 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153270pDGE479 M4E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153271pDGE498M5 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153272pDGE499M6 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153273pDGE500M6E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153274pDGE501M7 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153275pDGE502 M8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
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153276pDGE503Intermediate sgRNA array cloning vector position 1
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153277pDGE504 Intermediate sgRNA array cloning vector position 2
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153278pDGE505 Intermediate sgRNA array cloning vector position 3
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153279pDGE506Intermediate sgRNA array cloning vector position 4
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153280pDGE508End-linker for cloning 2 sgRNA arrays into any recipient vector
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153281pDGE509End-linker for cloning 3 sgRNA arrays into any recipient vector
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153282pDGE510End-linker for cloning 4 sgRNA arrays into any recipient vector
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Recombinant Antibodies from Article