CRISPRi-mediated Programming Essential Gene can as a Direct Enzymatic Performance Evaluation & Determination (DEPEND) System.
Tan SI, Yu PJ, Ng IS
Biotechnol Bioeng. 2020 May 27. doi: 10.1002/bit.27443.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154306 | pSUI-SynP-sfGFP | Expression of sfGFP under Synthetic promoter (SynP) and cloning of the sgRNA with simple screening of positive clone by fluorescence |
| 154307 | pSM-dCas9::Ap | Shuttle vector for expression dCas9, mobilization cluster, expresses the tracrRNA and a CRISPR array designed for the easy cloning of new spacers. |
| 154341 | pSUI-SynP-CAS1sg | Expression of the sgRNA targeting to the E. coli can gene |
| 154342 | pSUI-SynP-CAS2sg | Expression of the sgRNA targeting to the E. coli can gene |
| 154343 | pSUI-SynP-CANTsg | Expression of the sgRNA targeting to the E. coli can gene |
| 154344 | pSUI-SynP-CAPsg | Expression of the sgRNA targeting to the E. coli can gene |