Peroxisome compartmentalization of a toxic enzyme improves alkaloid production.
Grewal PS, Samson JA, Baker JJ, Choi B, Dueber JE
Nat Chem Biol. 2020 Oct 12. pii: 10.1038/s41589-020-00668-4. doi: 10.1038/s41589-020-00668-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158587 | pPSG465 | Enzymes Bv-CYP76AD5, DODC, ARO4_K229L |
| 158588 | pPSG569 | tNCS-ePTS1 Cen6/ARS4 |
| 158589 | pSJD001 | Pex22(1-36)-mRuby2 peroxisome marker |
| 158590 | pPSG1066 | tNCS-Venus-ePTS1 CEN6/ARS4 |
| 158591 | pJAS1643 | tNCS-VioE CEN6/ARS4 |
| 158592 | pJAS1644 | tNCS-VioE-dead_ePTS1 CEN6/ARS4 |
| 158593 | pJAS1668 | VioE-ePTS1 CEN6/ARS4 |
| 158594 | pJAS1669 | VioE-dead_ePTS1 CEN6/ARS4 |
| 158595 | pPSG1059 | tNCS-ePTS1, 6OMT, 4'OMT2, CNMT (CEN6/ARS4) |
| 158596 | pPSG1060 | tNCS-dead-ePTS1, 6OMT, 4'OMT2, CNMT (CEN6/ARS4) |
| 158597 | pPSG1067 | tNCS-Venus (CEN6/ARS4) |
| 158598 | pJAS1052 | VioA, VioB |
| 158599 | pJJB207 | UbiY-Venus-ePTS1 |
| 158600 | pJJB170 | Transcription factors ADR1c, PIP2c, OAF1c |
| 158601 | pPSG830 | tNCS-ePTS1 (2micron) |