Structural Basis for Helicase-Polymerase Coupling in the SARS-CoV-2 Replication-Transcription Complex.
Chen J, Malone B, Llewellyn E, Grasso M, Shelton PMM, Olinares PDB, Maruthi K, Eng ET, Vatandaslar H, Chait BT, Kapoor TM, Darst SA, Campbell EA
Cell. 2020 Sep 17;182(6):1560-1573.e13. doi: 10.1016/j.cell.2020.07.033. Epub 2020 Jul 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159092 | pCDFDuet-nsp7-nsp8 | Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8 |
| 159107 | pRSFDuet-sumo-NSP12 (SARS-CoV-2) | The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1). |
| 159390 | pET28a 6xHis PreScission SARS-CoV-2 nsp13 | Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site. |