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Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive beta Cell Destruction.
Ma H, Jeppesen JF, Jaenisch R
Cell Rep Med. 2020 Sep 22;1(6):100097. doi: 10.1016/j.xcrm.2020.100097. eCollection 2020 Sep 22.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
159280AAVS1-PDL1Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven human PDL1/CD274 (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.
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159281CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vectorA multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)
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159283CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNAA multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).
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159348INS-2A-luciferase-2A-tdTHuman INS targeting vector for knockin of 2A separated luciferase and tdTomato. Targeted cells will be puromycin resistant.
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159349INS-2A-luciferase-2A-CD19-GFPHuman INS targeting vector for knockin of 2A separated luciferase, humand CD19, and GFP. Targeted cells will be puromycin resistant.
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Antibodies from Article