Chemogenetic System Demonstrates That Cas9 Longevity Impacts Genome Editing Outcomes.

Sreekanth V, Zhou Q, Kokkonda P, Bermudez-Cabrera HC, Lim D, Law BK, Holmes BR, Chaudhary SK, Pergu R, Leger BS, Walker JA, Gifford DK, Sherwood RI, Choudhary A
ACS Cent Sci. 2020 Dec 23;6(12):2228-2237. doi: 10.1021/acscentsci.0c00129. Epub 2020 Nov 18. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
162723	pX330 NL-FKBP-Cas9	Mammalian expression
162724	pX330 LC-FKBP-Cas9	Mammalian expression
162725	pX330 NC-FKBP-Cas9	Mammalian expression
162726	pX330 NLC-FKBP-Cas9	Mammalian expression
162727	pX330 N-FKBP-Cas9	Mammalian expression
162728	pX330 L-FKBP-Cas9	Mammalian expression
162729	pX330 C-FKBP-Cas9	Mammalian expression
162730	pET SUMO NL-FKBP-Cas9	Bacterial expression, protein expression
162735	pU6 SgRNA RBM20	Mammalian expression
162736	pU6 SgRNA GAPDH	Mammalian expression