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High-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector.
Sinegubova MV, Orlova NA, Kovnir SV, Dayanova LK, Vorobiev II
PLoS One. 2021 Feb 2;16(2):e0242890. doi: 10.1371/journal.pone.0242890. eCollection 2021. PubMed Article

Plasmids from Article

ID Plasmid Purpose
162782p1.1-Tr2-eGFPFluorescent reporter for protein expression studies
162783pTMProtein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are coded
162785pTM-RBDv2SARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cells

Antibodies from Article