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Recording of elapsed time and temporal information about biological events using Cas9.
Park J, Lim JM, Jung I, Heo SJ, Park J, Chang Y, Kim HK, Jung D, Yu JH, Min S, Yoon S, Cho SR, Park T, Kim HH
Cell. 2021 Jan 28. pii: S0092-8674(21)00014-3. doi: 10.1016/j.cell.2021.01.014.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
164124Lenti_stgRNA-PuroBackbone for lentiviral stgRNA library construction (library 1 and 2)
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164131pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFPExpresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)
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164132pcDNA™5/FRT/CMV_promoter-ciCas9-E2A-mRFPExpresses ciCas9 and mRFP in mammalian cells (an insertion vector to generate ciCas9-knockin cell lines)
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164133Lenti_CMV-Cas9-P2A-HygRLentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells
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164134Lenti_EFS-Cas9-P2A-HygRLentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells
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164135Lenti_PGK-Cas9-P2A-HygRLentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells
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164136Lenti-pHSE::Cre-mRuby3Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction
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164137Lenti-pNFkB::Cre-mRuby3Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFa
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164138Cre-responsive Cas9 expressing transposon library vectorBackbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)
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