Overexpression of Key Sterol Pathway Enzymes in Two Model Marine Diatoms Alters Sterol Profiles in Phaeodactylum tricornutum.
Jaramillo-Madrid AC, Abbriano R, Ashworth J, Fabris M, Pernice M, Ralph PJ
Pharmaceuticals (Basel). 2020 Dec 21;13(12). pii: ph13120481. doi: 10.3390/ph13120481.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 139938 | AC_pTpEF2 | Diatom promoter |
| 139945 | CD_bsd | Resistance gene |
| 139946 | CD_nat | Resistance gene |
| 139948 | CF_OriT | Origin of transfer for conjugation cargo plasmids |
| 139949 | DE_Thr6XHisFLAG | Protein tags |
| 139951 | EF_tTpEF2 | Diatom terminator |
| 139961 | pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAG | Over-expression of heterologus squalene epoxidase from Nannochloropsis oceanica |
| 139963 | pL2-1 ConjEpi PtEF2bsd Pt49202HMGRVenusThrFLAG | Over-expression of native 3-hydroxy-3-methyl glutaryl CoA reductase |
| 139964 | pL2-1 ConjEpi PtEF2bsd Pt49202tHMGRVenusThrFLAG | Over-expression of N-terminal truncated version of native 3-hydroxy-3-methyl glutaryl CoA reductase |