Bim escapes displacement by BH3-mimetic anti-cancer drugs by double-bolt locking both Bcl-XL and Bcl-2.
Liu Q, Osterlund EJ, Chi X, Pogmore J, Leber B, Andrews DW
Elife. 2019 Mar 12;8. pii: 37689. doi: 10.7554/eLife.37689. PubMed Article
Liu Q, Osterlund EJ, Chi X, Pogmore J, Leber B, Andrews DW
Elife. 2019 Mar 12;8. pii: 37689. doi: 10.7554/eLife.37689. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166733 | Venus-Bad-pEGFP-C1 | Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad |
| 166734 | Venus-BimL-pEGFP-C1 | Express Venus fused to the N-terminus of the Bcl-2 family protein, BimL |
| 166735 | Venus-BimEL-pEGFP-C1 | Express Venus fused to the N-terminus of the Bcl-2 family protein, BimEL |
| 166736 | Venus-tBid-pEGFP-C1 | Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid |
| 166740 | Venus-Bad-4E-pEGFP-C1 | Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation in BH3 domain to disrupt binding anti-apoptotic proteins |
| 166741 | Venus-BimL-4E-pEGFP-C1 | Express Venus fused to the N-terminus of the Bcl-2 family protein, BimL with mutation in BH3 domain to disrupt binding anti-apoptotic proteins |
| 166742 | Venus-BimEL-4E-pEGFP-C1 | Express Venus fused to the N-terminus of the Bcl-2 family protein, BimEL with mutation in BH3 domain to disrupt binding anti-apoptotic proteins |
| 166748 | Venus-Bad-2A-pEGFP-C1 | Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation to 2 positions in the BH3 domain |
| 166749 | mCerulean3-Bcl-XL-s2193 | Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines. |
| 166750 | mCerulean3-Bcl-2-s2193 | Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2 in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines. |
| 166758 | Venus-BimEL(Bad)-pEGFP-C1 | To examine effect of swapping the BH3 region in full length BimEL with that of Bad |
| 177408 | mCerulean3-BclXL-pEGFP-C1 | To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL. |
| 177416 | mCerulean3-Bcl2-pEGFP-C1 | To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2. |