Computational redesign of a fluorogen activating protein with Rosetta.
Bozhanova NG, Harp JM, Bender BJ, Gavrikov AS, Gorbachev DA, Baranov MS, Mercado CB, Zhang X, Lukyanov KA, Mishin AS, Meiler J
PLoS Comput Biol. 2021 Nov 8;17(11):e1009555. doi: 10.1371/journal.pcbi.1009555. eCollection 2021 Nov.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 168475 | pET11a-Z-N-DiB-RM | N-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain DiB-RM‐split-Zip protein |
| 168476 | pET11a-N-DiB-RM | N-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain DiB-RM‐split protein |
| 168477 | pMRBad-Z-C-DiB-RM | C-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168475: pET11a-Z-N-DiB-RM to obtain DiB-RM‐split-Zip protein |
| 168478 | pMRBad-C-DiB-RM | C-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain DiB-RM‐split protein |
| 168782 | LifeAct-TagBFP-DiB-RM | Actin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RM |
| 168783 | Vimentin-DiB-RM | Vimentin - DiB-RM fusion protein |