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CRISPR-Mediated Transcriptional Repression in Toxoplasma gondii.
Markus BM, Boydston EA, Lourido S
mSphere. 2021 Oct 13:e0047421. doi: 10.1128/mSphere.00474-21. PubMed Article

Plasmids from Article

ID Plasmid Purpose
171088pU6-Universal.Sth.dCas9CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
171089pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
171090pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

Antibodies from Article