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(Plasmid #171088)


Item Catalog # Description Quantity Price (USD)
Plasmid 171088 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 2643
  • Total vector size (bp) 6285
  • Vector type
    Toxoplasma gondii expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    dam-/dcm- (NEB catalog # C2925I)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
  • Species
    Synthetic; Streptococcus thermophilus, Toxoplasma gondii
  • Insert Size (bp)
  • Promoter U6 (Toxoplasma gondii)

Cloning Information for Gene/Insert 1

  • Cloning method Unknown
  • 5′ sequencing primer gcgggacagagttgtctcgac
  • 3′ sequencing primer gcgggacagagttgtctcgac
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
  • Species
    Toxoplasma gondii
  • Insert Size (bp)
  • Mutation
    Note: A S245F mutation is present but did not appear to affect drug selection with pyrimethamine
  • Promoter DHFR-TS (Toxoplasma gondii)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NsiI (not destroyed)
  • 3′ cloning site SbfI (not destroyed)
  • 5′ sequencing primer gcgggacagagttgtctcgac
  • 3′ sequencing primer tctagaactagtggatc
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This construct was adapted from a previously published plasmid by Saima Sidik et al. (Addgene plasmid # 52694). The sgRNA scaffold was cloned from another published plasmid, which was kindly provided to us by Jeremy Rock and Sarah Fortune (Rock JM, Hopkins FF, Chavez A, et al. Programmable transcriptional repression in mycobacteria using an orthogonal CRISPR interference platform. Nat Microbiol. 2017;2:16274. Published 2017 Feb 6. doi:10.1038/nmicrobiol.2016.274).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-Universal.Sth.dCas9 was a gift from Sebastian Lourido (Addgene plasmid # 171088 ; ; RRID:Addgene_171088)
  • For your References section:

    CRISPR-Mediated Transcriptional Repression in Toxoplasma gondii. Markus BM, Boydston EA, Lourido S. mSphere. 2021 Oct 13:e0047421. doi: 10.1128/mSphere.00474-21. 10.1128/mSphere.00474-21 PubMed 34643425